The present textbook on Essentials of Practical Biochemistry has been prepared in user-friendly manner according to the requirements of medical, dental and paramedical students. The textbook has been written in simple language and provides all the relevant and essential information on various practical exercises that are required to be performed at undergraduate level. This textbook includes practical exercises on Qualitative Tests for Carbohydrates, Proteins, Amino Acids, Lipids and Non-protein Nitrogenous Substances, Qualitative and  Quantitative Analysis of Urine, and of various Body Fluids, Quantitative Estimation of various metabolites like glucose, cholesterol, urea, creatinine, triglycerides, electrolytes, uric acid, albumin, etc. in serum/plasma, estimation of various Serum Diagnostic Enzymes, Organ Function Tests and Biochemical Techniques. For quick and easy understanding, the practical exercises have been described in several headings like Introduction, Principle, Materials/ Apparatus required, Procedure, Observations, Calculation and Result. In each practical exercise, blank space has been provided for students to write down their own observations, readings and calculations then and there in the laboratory itself on the same day. Therefore, present textbook can also be used as a practical manual as well while working in laboratory. Normally, students find difficulty in understanding the procedure and calculation portions of the quantitative practical exercises. To overcome this problem, procedure and calculation portions of most exercises have been explained in an easy way with examples and space for calculation has been provided for students for their practice. For practice before examination,  Test Exercises and Model Papers have also been provided. A list of reagents and solutions along with the procedures of their preparation and normal biochemical values required for routine clinical practice have also been given in present textbook.